FIG. 3.

(A) Identification of NotI linking clones. Cosmid clones hybridizing with probes generated from the ends of NotI-digested V. cholerae genomic DNA were digested with BamHI (lanes a) and BamHI and NotI (lanes b). (B) Southern blot hybridization of PFGE-separated NotI- and CeuI-digested V. cholerae 569B genomic DNA with NotI linking cosmid clones A1044, A606, and A793 as probes. The linked NotI and CeuI fragments are marked. The clone A793, having no CeuI site, hybridized only with CeuI fragment C2.