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. 2023 Dec 4;32:09636897231215233. doi: 10.1177/09636897231215233

Figure 1.

Figure 1.

Immunofluorescent characterization of swine small intestine ENS. (A) Segment of swine small intestine was harvested and processed for H&E (B) and immunohistochemistry, including PGP9.5 (C), Hu/SMA (D, E), and Hu/P75 (F). Wholemount immunostaining of the myenteric plexus with Hu and calretinin reveals the myenteric ganglia with interconnecting nerve fibers (G). High-power images of myenteric ganglia show enteric neurons (PGP9.5, Hu) and glia (S100β, P75) (I, J–J’’’). Scale bars: A, 1 cm; G, 1 mm; H, 500 µm; E, 200 µm; C, D, F, I, and J, 100 µm. CM: circular muscle; ENS: enteric nervous system; GFP: green fluorescent protein; LM: longitudinal muscle; SMA: smooth muscle actin.