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. 2023 Dec 4;32:09636897231215233. doi: 10.1177/09636897231215233

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Optimizing culture conditions for swine ENSCs. (A) ENSCs cultured in 5% O₂ conditions formed significantly more enteric neurospheres than those grown in control conditions (20% O₂) or in the presence of various other putative growth factors. No change was observed in neurosphere size (B). Neurospheres grown in 5% O₂ exhibit significantly increased levels of Nestin transcript and decreased expression of SMA, relative to transcript levels in freshly isolated cells (C, *P < 0.05). RS67506: 5HT₄ agonist. ENSC: enteric neuronal stem cell; SMA: smooth muscle actin.