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. 2023 Dec 4;32:09636897231215233. doi: 10.1177/09636897231215233

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Identification of transplanted ENSCs and their differentiation within the rectum. (A) Rectum was explanted immediately following EUS-guided colonoscopic injection of multiple colored dyes into the rectal wall: green dye (green arrows), yellow dye (yellow arrows), and black ink (white arrows and circle). High-power imaging identified the injected RFP beads (B). Sections of recipient gut reveal successful targeting of the submucosa and muscularis layers with dye (C), and identification of GFP+ cells (D, green arrows) and RFP beads (D, white arrows) within the submucosal layer. Immunohistochemistry shows transplanted GFP+ ENSCs give rise to neurons (Tuj1; E, G, and H, arrows; arrowheads indicate endogenous plexus) and glial cells (F, S100β). High-power images of white dotted boxes in E are shown in (G and H). ENSC: enteric neuronal stem cell; EUS: endoscopic ultrasound; RFP: red fluorescent protein.