Skip to main content
. 2005 Mar;17(3):859–875. doi: 10.1105/tpc.104.029629

Figure 3.

Figure 3.

Analyses of Expression of GhACT Genes in Cotton Tissues.

(A) Real-time RT-PCR analysis of expression of GhACT genes in cotton tissues. Relative value of GhACT gene expression in cotton tissues, including leaf (1), stem (2), cotyledon (3), root (4), anther (5), fiber (6), and petal (7), was shown as percentage of GhUBI expression activity (see Methods).

(B) RNA gel blot analysis of GhACT1 transcripts in cotton. Total RNA (20 μg/lane) from petal (1), anther (2), leaves (3), cotyledon (4), root (5), ovule (6 to 10) at 4, 8, 14, 21, and 28 DPA, and fiber (11 and 12) at 8 and 14 DPA was fractionated on a 1.2% denaturing agarose gel and transferred onto a nylon membrane (see Methods). Top panel, autoradiograph of RNA hybridization; bottom panel, RNA gel before transfer to membrane showing equal loading of RNAs.