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Genomic DNA Gel Blot Analysis of the GhACT1 Gene.
Thirty micrograms of genomic DNA was digested with restriction enzymes as indicated and fractionated on a 0.8% agarose gel. DNA gel blots were hybridized with 32P-labeled GhACT1 5′-region gene-specific probe (0.8 kb) (A) and 32P-labeled GhACT1 exon 3 probe (0.6 kb) (B). B, BamHI; E, EcoRI; H, HindIII; X, XbaI; EV, EcoRV; S, SacI.
