Table 1.

Primers Used in Gene-Specific RT-PCR of GhACT Genes

Genes	Primers
GhACT1	5′-CCCTTGAATATTAAATAAATAAAAAAATA-3′
	5′-TTGTGCTCAGTGGGGGTTCAACC-3′
GhACT2	5′-TGCCCGGAAGTCCTCTTCCAG-3′
	5′-ATTTTCCCAGAAGTTTGACCGCGC-3′
GhACT3	5′-CCCTTGAATATTAAATAATAATAAGCAC-3′
	5′-TTGTGCTCAGTGGGGGTTCAACT-3′
GhACT4	5′-GGGGGAGCCTTGAATATGAAATTG-3′
	5′-TTGTGCTCAGTGGGGGTTCAACC-3′
GhACT5	5′-ATTTTCCCAGAAGTTTGACCGCGC-3′
	5′-TGCCCGGAAGTCCTCTTCCAA-3′
GhACT7	5′-TTAAAGAAAATATAAGAAATAAGCATCA-3′
	5′-GTATGCCAGTGGTCGGACGACA-3′
GhACT8	5′-TTAAAGAAAATATAAGAAATAAGCATCA-3′
	5′-GTATGCCAGTGGTCGGACGCAG-3′
GhACT9	5′-ATCTTCAACATAAAAGATCATCCCACT-3′
	5′-GATCTATCTTGGCATCACTCAGCA-3′
GhACT10	5′-AACCAGATATTAAATATAATTTCCGTAG-3′
	5′-GGGAAATTGTCCGTGACATGAAG-3′
GhACT11	5′-ACAATAGCTATTGACATTAATGTTTGC-3′
	5′-TTGTGCTCAGTGGGGGTTCAACT-3′
GhACT12	5′-AACCAGATATTAAATATAATTTCCGTAG-3′
	5′-GGGAAATTGTCCGTGACATGAAA-3′
GhACT13	5′-CCCTTGAATATTAAATAATAATAAGCAC-3′
	5′-TTGTGCTCAGTGGGGGTTCAACC-3′
GhACT14	5′-AACCAGATATTAAATATAATTTCCGTAA-3′
	5′-ATTGGAGCTGAGAGATTCCGTTG-3′
GhACT15	5′-ATCTTCAACATAAAAGATCATCCCACT-3′
	5′-GATCTATCTTGGCATCACTCAGCG-3′
GhUBI	5′-CTGAATCTTCGCTTTCACGTTATC-3′
	5′-GGGATGCAAATCTTCGTGAAAAC-3′

The efficiency of each primer pair was detected using GhACT cDNA clones as standard templates, and the RT-PCR data were normalized with the relative efficiency of each primer pair.