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. 2005 Mar;17(3):888–902. doi: 10.1105/tpc.104.028829

Figure 2.

Figure 2.

Inhibition of the Vacuolar Trafficking of AALP:GFP and Spo:GFP by Lat B.

(A) Phenotypic analysis of the effect of Lat B on AALP:GFP and Spo:GFP localization. Protoplasts transformed with AALP:GFP or Spo:GFP were incubated in the presence [Lat B (+); panels b to d and g to k] and absence [Lat B (−); panels a, e, and f] of Lat B (10 μM), and the localization of AALP:GFP and Spo:GFP was examined 24 and 48 h after transformation, respectively. The red images show chlorophyll autofluorescence. CH, chloroplasts. Bar = 20 μm.

(B) Quantification of the Lat B–altered distribution patterns of Spo:GFP. Protoplasts were counted based on the Spo:GFP distribution patterns in the presence [Lat B (+)] and absence [Lat B (−)] of Lat B (10 μM) 24 and 48 h after transformation. In each experiment, >100 transformed cells were counted, and three independent experiments were performed to obtain means and sd. Representatives of the ER (network pattern), punctate staining, and vacuole patterns of Spo:GFP are shown in panels g, i, and k of Figure 2A, respectively. Note that a small fraction of protoplasts showing the punctate staining pattern together with the ER pattern or the vacuolar pattern was counted as the punctate staining pattern because the punctate staining pattern was considered to reflect the inhibition of the vacuolar trafficking of Spo:GFP.