Figure 2.

Accumulation of p1 Transcripts in P1-rw1077 and P1-rr4B2 during Development.

(A) RNA gel blot analysis of p1 transcripts of P1-rw1077, P1-rr4B2, and p1-ww1112 at different developmental stages. Samples were taken from whole ears (0 DAP), developing kernels and cob (2 to 6 DAP), whole kernel (8 DAP), and kernel pericarp (12 to 21 DAP). Ten micrograms of total RNA was loaded in each lane. Hybridization probes are indicated at right. The actin cDNA probe was used as a loading control.

(B) Quantified RNA levels of p1 and a1 from the RNA gel blot in (A) are normalized to actin to calculate relative transcript levels (y axis).