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. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: J Vis Exp. 2022 May 2;(183):10.3791/61277. doi: 10.3791/61277

Figure 3.

Figure 3.

Step-by-step protocol for unopened aorta Oil Red O staining and imaging. (A) Pin the whole aorta with branches on a wax petri dish. (B) Cover the aorta with Oil Red O staining solution. (C) Image illustrate the whole aorta after Oil Red O staining. (D) Image illustrate Oil Red O stained whole aorta after cleaning. (E) Representative photomicrographs of Oil Red O stained whole aorta of TGFβR2iSMC-Apoe mice after 4 months of HCHF diet. (a’) A high-magnification of ascending aorta from a. (b’) A high-magnification of abdominal aorta from b.