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The Corresponding cDNAs for Nine Consecutive Enzymes Involved in Protoberberine Alkaloid Biosynthesis Have Been Isolated.
TYDC, tyrosine/dopa decarboxylase; 6OMT, (S)-norcoclaurine-6-O-methyltransferase; CNMT, (S)-coclaurine N-methyltransferase; CYP80B, (S)-N-methylcoclaurine-3′-hydroxylase; 4′OMT, (S)-3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase; BBE, berberine bridge enzyme; SOMT, (S)-scoulerine-9-O-methyltransferase; CYP719A, (S)-canadine synthase.
