Figure 3.

Protoberberine Alkaloid Biosynthetic Gene Transcripts Are Localized to the Immature Endodermis and Surrounding Tissues in T. flavum Roots.

(A) to (R) In situ hybridization using DIG-labeled antisense probes for TYDC ([A] and [B]), NCS ([C] and [D]), 6OMT ([E] and [F]), CNMT ([G] and [H]), CYP80B ([I] and [J]), 4′OMT ([K] and [L]), BBE ([M] and [N]), SOMT ([O] and [P]), and CYP719A ([Q] and [R]) performed on cross ([A], [C], [E], [G], [I], [K], [M], [O], and [Q]) and longitudinal ([B], [D], [F], [H], [J], [L], [N], [P], and [R]) root sections.

(S) and (T) In situ hybridization using a DIG-labeled sense probe for CYP719A performed on cross (S) and longitudinal (T) root sections. Bar = 50 μm for (A) to (T).