Figure 4.

Protoberberine Alkaloid Biosynthetic Gene Transcripts Are Localized to the Protoderm of Leaf Primordia in T. flavum Rhizomes.

(A) Rhizome longitudinal section stained with toluidine blue O.

(B) to (K) In situ hybridization using DIG-labeled antisense probes for TYDC (B), NCS (C), 6OMT ([D] and [K]), CNMT (E), CYP80B (F), 4′OMT (G), BBE (H), SOMT (I), and CYP719A (J) performed on longitudinal ([B] to [J]) and cross (K) rhizome sections.

(L) In situ hybridization using a DIG-labeled sense probe for CYP719A performed on a longitudinal rhizome section.

Yellow and red asterisks in (A) show the location of leaf primordia and axillary buds, respectively. Bar = 250 μm in (A) and 50 μm in (L). Bar in (L) also applies to (B) to (K).