Table 1.
Similarity between Predicted Protein Sequences of cDNAs Isolated from a T. flavum Cell Suspension Culture Library and Benzylisoquinoline and Protoberberine Alkaloid Biosynthetic Enzymes from Related Species
| Enzyme | Plant Species | Identity (%) | Homology (%) | Accession |
|---|---|---|---|---|
| TYDC | Papaver somniferum | 79 | 89 | U08598 |
| NCS | Thalictrum flavum | – | – | AY376412 |
| 6OMT | Coptis japonica | 85 | 92 | D29811 |
| Papaver somniferum | 65 | 80 | AY268894 | |
| CNMT | Coptis japonica | 85 | 92 | AB061863 |
| Papaver somniferum | 62 | 81 | AY217336 | |
| CYP80B | Coptis japonica | 84 | 90 | AB025030 |
| Papaver somniferum | 68 | 84 | AF134590 | |
| Eschscholzia californica | 66 | 83 | AF014801 | |
| 4′OMT | Coptis japonica | 88 | 92 | D29812 |
| Papaver somniferum | 67 | 81 | AY217334 | |
| BBE | Berberis stolonifera | 68 | 82 | AF049347 |
| Papaver somniferum | 58 | 77 | AF025430 | |
| Eschscholzia californica | 57 | 76 | S65550 | |
| SOMT | Coptis japonica | 89a | 95a | D29809 |
| CYP719A | Coptis japonica | 86 | 93 | AB026122 |
a
Alignment does not include sequences corresponding to a putative extension of 30 amino acids at the N terminus of C. japonica SOMT, which are found in T. flavum SOMT.