Figure 7.

acx1 Plants Are Compromised in JA-Mediated Resistance to Tobacco Hornworm Larvae.

(A) Newly hatched hornworm larvae were allowed to feed on 4-week-old wild-type and acx1 plants for 3 d. Total RNA was isolated separately from hornworm-damaged (D) and undamaged (U) leaves of the same genotype. RNA was also isolated from unchallenged plants as a control (C). RNA gel blots were hybridized to cDNA probes for the genes indicated on the left.

(B) Three-week-old wild-type (closed bar) and acx1 (open bar) plants were challenged for ∼4 h with third-instar tobacco hornworm larvae. JA was extracted from damaged leaf tissue (+THW) and from leaves of unchallenged plants (control) and quantified by GC-MS. Data show the mean ± sd of three independent JA extractions.

(C) Representative wild-type plants at the end of experiment 1 feeding trial.

(D) Representative acx1 plants at the end of experiment 1 feeding trial.

(E) Hornworm larvae recovered from representative wild-type (left column) and acx1 (right column) plants at the end of experiment 1 feeding trial.