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. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: Cell Rep. 2023 Oct 31;42(10):113297. doi: 10.1016/j.celrep.2023.113297

Figure 2. Noni FAs evoke species-specific feeding preferences.

Figure 2.

(A) Diagram of binary feeding assay used for (B)–(D) and (F)–(H) outcomes. Created with BioRender.com.

(B–D) Feeding preference of indicated species for mixtures of 5 mM sucrose with varying concentrations of octanoic acid (OA), hexanoic acid (HA), or decanoic acid (DA) tested against 1 mM sucrose. n = 12 trials for each concentration of the indicated FA.

(E) Flies used for results shown in (F)–(H): Canton-S (wild-type), orco1 (∆orco), or orco1 with the third segment of the antennae removed surgically (∆orco antennae-less). Created with BioRender.com.

(F–H) Feeding preference of indicated flies for 5 mM sucrose with varying concentrations of OA (F, n = 12–14), HA (G, n = 11–12), or DA (H, n = 11–12) tested against 1 mM sucrose. Data were analyzed using two-way ANOVA with Tukey’s post hoc multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars represent SEM.