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. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: Cell Rep. 2023 Oct 31;42(10):113297. doi: 10.1016/j.celrep.2023.113297

Figure 5. Noni FAs modulate sugar preference in a species-specific manner.

Figure 5.

(A) Diagram of binary feeding assay used for (B)–(G) and outcomes. Created with BioRender.com.

(B) Feeding preference of named species to 2 mM sucrose mixed with varying concentrations of octanoic acid (OA) tested against 2 mM sucrose alone. n = 12. (B’) D. sechellia results from (B), with preference index (PI) for each sucrose-OA mixture tested for significance from zero.

(C) Feeding preference of named species to 2 mM sucrose mixed with varying concentrations of hexanoic acid (HA) tested against 2 mM sucrose alone. n = 12. (C’) D. sechellia results from (C), with preference index (PI) for each sucrose-HA mixture tested for significance from zero.

(D) Feeding preference of named species to 2 mM sucrose mixed with varying concentrations of decanoic acid (DA) tested against 2 mM sucrose alone. n = 12.

(D’) D. sechellia results from (D), with preference index (PI) for each sucrose-DA mixture tested for significance from zero.

(E–G) Feeding preference of D. sechellia, tested intact (D sech) or with antennae surgically removed (D sech antennae-less), for 2 mM sucrose mixed with varying concentrations of OA (E), HA (F), or DA (G) tested against 2 mM sucrose alone. n = 9 (OA), n = 10 (HA), n = 10 (DA). Data in (B)–(G) were analyzed using two-way ANOVA with Tukey’s post hoc multiple comparisons test. Data in (B’)–(D’) were tested for significance from hypothetical mean 0 using the Wilcoxon test. For all graphs, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars represent SEM.