FIG. 3.

Changes in methylation of Tar and hybrid receptors in cells exposed to attractants. Approximately equal amounts of total protein extracted from cells of strain VB13 (ΔT) were loaded in each lane. The samples in the left three lanes were from cells containing plasmid pMK113 (tar⁺), the samples in the middle three lanes were from cells containing plasmid pTarp, and the samples in the right three lanes were from cells containing plasmid pTapr and induced with 1 mM IPTG. Within each set of three lanes, the sample loaded on the left was from unstimulated cells, the sample loaded in the middle was from cells exposed and adapted to 1 mM Pro-Leu, and the sample loaded on the right was from cells exposed and adapted to 10 mM l-aspartate. The proteins were visualized after incubation with antiserum against the entire E. coli wild-type Tar protein. SDS-PAGE was carried out with an 8% acrylamide gel.