FIG. 4.

Immunoblot analysis of hybrid and engineered receptors with TsrCSR antiserum. Samples containing approximately equal amounts of protein were loaded in each lane. Samples came from cells of strain VB13 containing the following plasmids (from left to right): pTapl, pTapr, pSW2 (tap⁺), pTart, pTarp, and pMK113 (tar⁺). Cultures of cells containing pTapl, pTapr, and pSW2 contained 1 mM IPTG. The proteins were visualized after incubation with the TsrCSR antiserum. SDS-PAGE was carried out with a 10% acrylamide gel.