FIG. 5.

Immunoblot analysis of hybrid and engineered receptors with Trg antiserum. Samples containing approximately equal amounts of protein were loaded in each lane. Samples came from cells of strain VB13 containing the following plasmids (from left to right): pTapl, pTapr, pSW2 (tap⁺), pTart, pTarp, and pMK113 (tar⁺). Cultures of cells containing pTapl, pTapr, and pSW2 contained 1 mM IPTG. The proteins were visualized after incubation with antiserum against the entire E. coli wild-type Trg protein. SDS-PAGE was carried out with an 8% acrylamide gel.