TABLE 1.
Primers/probe | Sequence 5′-3′ | Annealing temperature (°C) | Product size (bp) |
---|---|---|---|
pCS20 Sol1F | ACAAATCTGGYCCAGATCAC | 55 | 280 |
pCS20 Sol1 R | CAGCTTTCTGTTCAGCTAGT | - | - |
Sol1 TM probe | 6-FAM-ATCAATTCACATGAAACATTACATGCAACTGG-BHQ1 | - | - |
lipA F | GGATCCTCATGAGCCTCAAA | 62 | 430 |
lipA R | CTGCCGACCTTAAATCATCCATA | - | - |
lipB F | TGGAAGATTGAATCCTTACCTG | 59 | 452 |
lipB R | CCATGATATGTTATCCATTTTC | - | - |
secY F | CCAGGCATTAATCCAGATGT | 61 | 690 |
secY R | GCGGAACATATGTAGATGCAGT | - | - |
sodB F | TGCCAGAACTGCCTTATCAA | 61 | 507 |
sodB R | AAGCGTGTTCCCATACATCC | - | - |
sucA F | TGAAAGGCTTTGGCTACAGG | 59 | 566 |
sucA R | CCTGACCAATTACAGCAGCA | - | - |
bp, base pairs.