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. 2023 Nov 14;90(1):2119. doi: 10.4102/ojvr.v90i1.2119

TABLE 1.

Oligonucleotides used for pCS20 qPCR and polymerase chain reaction amplification of housekeeping genes.

Primers/probe Sequence 5′-3′ Annealing temperature (°C) Product size (bp)
pCS20 Sol1F ACAAATCTGGYCCAGATCAC 55 280
pCS20 Sol1 R CAGCTTTCTGTTCAGCTAGT - -
Sol1 TM probe 6-FAM-ATCAATTCACATGAAACATTACATGCAACTGG-BHQ1 - -
lipA F GGATCCTCATGAGCCTCAAA 62 430
lipA R CTGCCGACCTTAAATCATCCATA - -
lipB F TGGAAGATTGAATCCTTACCTG 59 452
lipB R CCATGATATGTTATCCATTTTC - -
secY F CCAGGCATTAATCCAGATGT 61 690
secY R GCGGAACATATGTAGATGCAGT - -
sodB F TGCCAGAACTGCCTTATCAA 61 507
sodB R AAGCGTGTTCCCATACATCC - -
sucA F TGAAAGGCTTTGGCTACAGG 59 566
sucA R CCTGACCAATTACAGCAGCA - -

bp, base pairs.