FIG. 2.

(A) β-Gal activity results of the five P_15A10 regions subcloned into the promoter screening vector pTRK390. Time 0 is immediately before the addition of phage φ31 (cells at OD₆₀₀ of ≅0.5). β-Gal assays were performed at least three different times. For each assay, time point determinations were performed in duplicate. (B) Northern analysis of RNA hybridized with a ³²P-labeled lacZ.st probe. Northern analysis was not performed on subclones 1–305 and 442–574 because phage-inducible activity was associated exclusively with start sites 703 and 744.