FIG. 7.

Results of gel retardation assays performed with the ORF2 gene product. The labeled fragments used in the gel retardation assay are indicated at the top of the gels. For each fragment, lane 1 represents the control (no ORF2 added), lane 2 represents the effects of nonlabeled, competitive DNA on DNA binding (unlabeled subclone 588–888 added with ORF2), and lane 3 represents the ability of ORF2 to bind each fragment with no unlabeled 566–888 fragment present. The arrows to the right of each panel indicate the shift in mobility of each fragment upon addition of ORF2. For all three fragments, the ORF2 gene product was added from the same in vitro transcription-translation tube to ensure that the amount was identical between reactions.