. 1998 Feb;180(4):921–931. doi: 10.1128/jb.180.4.921-931.1998

TABLE 1.

Bacterial strains and plasmids used in this study

Strains	Description^a	Reference or source
E. coli
JM110	E. coli cloning host	39
DH5α	E. coli cloning host	Gibco-BRL
MC1061	E. coli host for pNZ18	4
BIL21 (DE3)	Expression host for pET28a	Novagen
L. lactis subsp. lactis NCK203	Sensitive host for phage φ31	15
Plasmids
pT7Blue	Ap^r; T-vector for cloning PCR products	Novagen
pTRK390	Em^r; lactococcal promoter screening vector	27
pTRK391	Em^r; pTRK390::P_15A10	27
pTRK477	Em^r; pTRK390::P_566–888	This study
pTRK486	Em^r; pTRK390::P_566–862	This study
pTRK481	Em^r; pTRK390::P_566–849	This study
pTRK485	Em^r; pTRK390::P_566–792	This study
pTRK487	Em^r; pTRK390::P_566–732	This study
pTRK483	Em^r; pTRK390::P_566–888S	This study
pTRK482	Em^r; pTRK390::P_566–862S	This study
pTRK484	Em^r; pTRK390::P_{15A10/mutORF2}	This study
pET28a	Kn^r; E. coli expression vector (T7 based)	Novagen
pTRK478	Kn^r; pET28a::ORF2	This study
pNZ18	Cm^r; high-copy-number shuttle vector	8
pTRK452	Cm^r; pNZ18::P_566–888::ORF2	This study
pTRK479	Cm^r; pNZ18::P6::antiORF2::T_T7	This study
pTRK360	Cm^r, Em^r; pSA3 + ori31	26
pTRK392	Cm^r, Em^r; pTRK360::P_15A10:: lacZ.st	27
pTRK480	Cm^r, Em^r; pTRK360::P_566–862S:: lacZ.st	This study
pCITE4a	Ap^r; for in vitro transcription-translation	Novagen

Abbreviations: Ap^r, ampicillin resistance; Em^r, erythromycin resistance; Cm^r, chloramphenicol resistance; Kn^r, kanamycin resistance.