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Activation of P566–888::lacZ.st by ORF2 provided in trans in E. coli
| Constructs (in E. coli) | Time point (min) | Activity (β-Gal units) |
|---|---|---|
| pET28A plus P566–888 (control) | 0 | 69 |
| 60 | 172a | |
| 120 | 185a | |
| pET::ORF2 plus P566–888 | 0 | 73 |
| 60 | 380 | |
| 120 | 684 |
The increase in β-Gal activity in this case represents the stalling of growth caused by induction of E. coli BIL21 (DE3) with IPTG. β-Gal units are expressed per OD600 of the cell culture. Inhibition of growth causes a slight rise in β-Gal levels.
