Figure 3.

Functional analysis of actVA-3. (A) Phenotypic effect of the actVA3 deletion on ACT biosynthesis demonstrates reduced production of blue pigmented actinorhodin in the mutant strain in comparison to the wild type. Strains were inoculated on R5 solid medium and grown at 30°C for 3 days. The reverse side of plates is shown for (i) S. coelicolor M145ΔactVA-3 mutant and (ii) S. coelicolor M145 wild type. (B) HPLC chromatogram traces recorded at 520 nm of culture extracts from S. coelicolor M145 wild type (top) and S. coelicolor M145ΔactVA-3 mutant (bottom) demonstrates reduced production of actinorhodin in the mutant. (C) Chemical structure of γ actinorhodin and (D) UV/Vis spectrum of γ actinorhodin.