Fig. 4.

MeRIP-seq of whole adult male flies. A) t-SNE dimension reduction analysis based on 12,619 genes. B) Venn diagram displaying the common and unique methylated genes among groups. C) Annotation locations in genes of the significant (differential) m⁶A peaks. D) Proportions of the m⁶A methylated and DMGs with different m⁶A peak counts. E) m⁶A peak distribution across the mRNA meta-transcript, showing differences in enrichment around annotated locations. F) m⁶A read count frequency near TSSs. G) KEGG enrichment analysis based on the m⁶A DMGs identified in the single-factor comparisons between groups. Nine-quadrant diagrams showing that the m⁶A epitranscriptome and transcriptome are weakly negatively correlated (H) between the BD25 and DD25 flies and (I) between the DD25 and DD10 flies. A fold change ≥2 (1.5), P < 0.01, and an FDR < 0.01 were used as criteria for identifying significant (differential) m⁶A peaks.