Figure 1.

Differentially expressed miRNAs in DAn differentiation. a, miRNA expression during dopaminergic differentiation of epiSCs are represented as mean of the logarithmic ratio between DAn (SHH/FGF8) and Ctrl triplicate samples for both the differentiation time point DIV9 (x axis) and DIV14 (y axis). Only logFC values ≥ |0.5| were represented. For each miRNA, the bubble's color and size represent, respectively, the number of predicted target genes and the –Log(PValue) term enrichment in the KEGG pathway mmu04728:dopaminergic synapse. Targets were predicted using TargetScan 8.0 at default parameters. Red-circled miRNAs were selected for further analysis. b, Fold changes of miRNAs upregulated during the whole differentiation process (always-UP) or with a fluctuating expression (Discordant).