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. 2023 Nov 29;43(48):8104–8125. doi: 10.1523/JNEUROSCI.0431-23.2023

Figure 5.

Figure 5.

miR-218 is expressed in mesencephalic DA nuclei during mice development. a, b, TaqMan assay for the expression of miR-218 (a) and miR-9 (b) in FACS-purified GFP+ and GFP− cells from Th-GFP, Pitx3-GFP, and Lmx1a-GFP reporter mouse embryos at different developmental stages. Data are normalized to the average of the reference sno-202 and represent the mean ± SEM of 2−ΔCt values from three independent experiments. *p < 0.05 of GFP+ with respect to GFP− (two-way ANOVA followed by Sidak). c, ISH for miR-218 and IHC for Islet1, Pitx3, and Th (markers, respectively, of oculomotor and dopaminergic midbrain nuclei) in coronal section of brains from E14.5 mouse embryos. Consecutive sections were stained for the different markers (different lanes) while disposed with an anteroposterior order (on the same lane). Magnification for miR-218 ISH on DA nuclei is shown in the second lane. Scale bars: ISH, 75 μm; IF, 100 μm. Digital magnification is 4.2×. d, TaqMan assay for the expression of miR-218 in DAn differentiated from epiSCs (left); E12 neuronal progenitor cells (mE12-PCs) expressing the pro-dopaminergic transcription factors Nurr1 and Lmx1a (NL) (middle); and iDA cells transdifferentiated with the reprogramming cocktail ANL (Asl1, Nurr1, Lmx1a) (right). Data are normalized to the average of the reference sno-202 and represent the mean ± SEM of 2−ΔCt values from three independent experiments. *p < 0.05 of GFP+ with respect to GFP− (two-way ANOVA followed by Sidak).