Figure 9.
Deletion of miR-218 affects DA released. a–c, Analysis of IAHP. IAHP peak (a) and area (b) elicited by a 100 ms voltage command to 0 mV is significantly reduced in KO-1 and c-dKO compared with WT DAn (IAHP peak). KO-1, p < 0.01; c-dKO, p < 0.001; IAHP area, KO-1, p < 0.05; c-dKO, p < 0.001; unpaired Student t test. Data are mean ± SEM. c, Example traces of IAHP in WT, KO-1, and c-dKO DAn showing a clear dosage expression effect of miR-218 onto IAHP. d, Maximal concentration (µm) of DA released in response to single rectangular electrical pulse, recorded by CPA, is significantly reduced in KO1 and c-KO2. KO-1, p < 0.05; KO-2, p < 0.001; but not in c-dKO striatal slices (p = 0.067); unpaired Student t test. Data are mean ± SEM. Uptake system(s) parameters, such as half-life time (e) and latency (f) of amperometric current, are increased selectively in c-dKO compared with WT striata (p < 0.001 and p < 0.05, respectively; unpaired t test), indicating that the complete miR-218 deletion reduces DA uptake efficacy.