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. 2023 Oct 26;11(24):7856–7866. doi: 10.1039/d3bm00951c

Formulation ratios of different components used in formulation of LNPs.

Lipid nanoparticles Dye (mole ratio) DPPC (moles%) DOPC (moles%) DPPG (moles%) PEG-2000-AMINE (moles%) 5BDBD (mole ratio)
Lipo 47 47 0.5 5.5
Lipo + dye 1 : 500 47 47 0.5 5.5
Lipo + 5BDBD 47 47 0.5 5.5 1 : 100
Lipo + 5BDBD + dye 1 : 500 47 47 0.5 5.5 1 : 100