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. Author manuscript; available in PMC: 2023 Dec 5.
Published in final edited form as: Cell Rep. 2023 May 18;42(5):112513. doi: 10.1016/j.celrep.2023.112513

Figure 3. Identification of intra- and extravascular Syn Ly6c− cells by flow cytometry.

Figure 3.

(A) Annotation of CITE-seq data on C57BL/6 CD45+CD11b+Ly6G−SiglecF−CD64−MHCII− cells to subpopulations defined in total Syn Ly6c− (CD45+CD11b+Ly6G−SiglecF−CD64−) (Figure 2H).

(B) Expression of TR-MC (Lyve1), DC (CD74), cycling (Top2a), and monocyte (Plac8) subpopulation genes.

(C) Mean ADT intensity of surface markers.

(D) Annotation of scATAC-seq data on C57BL/6 CD45+CD11b+Ly6G−SiglecF−CD64−MHCII− cells to subpopulations defined in total Syn Ly6c− (Figure 2H).

(E and F) (E) Transcription factor (TF) activity and (F) expression of corresponding genes associated with TR-MCs (MafB and MYC), cycling (Fli1), and monocyte (Irf8) subpopulations.

(G) Gating strategy to distinguish intravascular NCMs vs. extravascular DCs and TR-MCs.

(H) NCMs, DCs, and TR-MCs in steady state.