FIG. 2.

SDS-polyacrylamide gel electrophoresis of outer membrane protein. The sarcosyl-insoluble envelope protein fraction was prepared as described in the legend to Fig. 1. Samples were electrophoresed on a 0.1% SDS–12.5% polyacrylamide gel. Protein was detected by staining with Coomassie brilliant blue R-250 (A) or by using antiserum against TolC (B). Lanes: M, molecular mass markers; 1, JA300; 2, OST3408; 3, OST3410; 4, JA300(pBluescript II); 5, JA300(pHA105); 6, JA300(pOST42BR); 7, JA300(pHc3R); 8, JA300T; 9, OST3408T; 10, JA300S; 11, JA300R. Arrows indicate the proteins discussed in the text.