TABLE 1.
Bacterial strains and plasmids used
| E. coli strain or plasmid | Genotype | Organic solvent tolerance | Reference or source |
|---|---|---|---|
| Strains | |||
| JA300 | F−thr leuB6 trpC1117 thi rpsL20 hsdS | n-Hexane | 21 |
| OST3408 | F−thr leuB6 trpC1117 thi rpsL20 hsdS marR08 | Cyclohexane | 1, 7 |
| OST3410 | F−thr leuB6 trpC1117 thi rpsL20 hsdS | Cyclohexane | 5 |
| OST3408Tc | F−thr leuB6 trpC1117 thi rpsL20 hsdS marR08 zde-234::Tn10 | Cyclohexane | 7 |
| JA300R | F−thr leuB6 trpC1117 thi rpsL20 hsdS marR08 zde-234::Tn10 | Cyclohexane | This study |
| JA300S | F−thr leuB6 trpC1117 thi rpsL20 hsdS zde-234::Tn10 | n-Hexane | This study |
| JA300T | F−thr leuB6 trpC1117 thi rpsL20 hsdS tolC | Decane | This study |
| OST3408T | F−thr leuB6 trpC1117 thi rpsL20 hsdS marR08 tolC | Decane | This study |
| CH1692 | topA57 argA supD74 rpsL Δ(lac-514) tolC::Tn10 | NTa | 11 |
| MC4100 | F−araD139 ΔlacU169 rpsL relA thi | NT | 23 |
| Plasmids | |||
| pHA105 | 0.4-kb AflII-PvuII fragment containing the marA gene in pBluescript II | 7 | |
| pOST42BR | 1.9-kb SalI-BamHI fragment containing the robA gene in pBluescript II | 33 | |
| pHc3R | 0.4-kb HincII fragment containing the soxS gene in pBluescript II | 34 | |
| pUX | pUC19 carrying the tolC gene | M. Wachi | |
| pMX | 2.5-kb EcoRI-HindIII containing the tolC gene in pMW119 | This study |
a
NT, not tested.