Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic parameters of different MmCDO and BsCDO species as determined by activity assays
| Sample | % Crosslinked In SDS-PAGE Gel |
KM (mM) | kcat (sec−1) | kcat/KM (mM−1sec−1) |
|---|---|---|---|---|
|
MmCDO Prep 2 |
Not measured | 5.5 ± 0.3 | 0.8 ± 0.1 | 0.15 ± 0.03 |
| BsCDO | 0 | 6.6 ± 0.8 | 0.4 ± 0.1 | 0.05 ± 0.02 |
| G82C BsCDO High Iron Prep 1 |
62 | 10.7 ± 0.3 | 0.17 ± 0.02 | 0.016 ± 0.002 |
| G82C BsCDO Low Iron Prep 2 |
34 | 11.5 ± 0.1 | 0.0041 ± 0.0003 | 0.00036 ± 0.00003 |
