Table 2. Sterility of drd knockdown females.
| Maternal genotype (# of eggs) | w; CyO/+; T155-GAL4/+ (153) | w; CyO/+; T155-GAL4/+ (221) | w; CY2-GAL4/CyO (238) | w; CY2-GAL4/CyO (163) |
|---|---|---|---|---|
| Collapsed (% of total) | 0% | 0.5% | 0.4% | 0.6% |
| Unfertilized (% of turgid eggs) | 3.4% | 10.3% | 2.2% | 4.5% |
| Pre-gastrulation (% of fertilized eggs) | 0.7% | 1.0% | 1.3% | 2.7% |
| Maternal genotype (# of eggs) | w; UAS-Dcr-2 drdGD3367/+; T155-GAL4/+ (188) | w; drdGD15915 UAS-Dcr-2/+; T155-GAL4/+ (155) | w; UAS-Dcr-2 drdGD3367/CY2-GAL4 (169) | w; drdGD15915 UAS-Dcr-2/CY2-GAL4 (206) |
| Collapsed (% of total) | 32.4%**** | 43.9%**** | 21.9%**** | 24.3%**** |
| Unfertilized (% of turgid eggs) | 6.1% | 10.0% | 7.8%* | 4.8% |
| Pre-gastrulation (% of fertilized eggs) | 100%**** | 98.6%**** | 100%**** | 96.4%**** |
Table 2: Embryonic arrest upon knockdown of drd expression in the follicle cells. Sibling control females (top four rows) and drd knockdown females (bottom four rows) were mated with sibling males, and eggs were collected and scored as in Table 1. The number of uncollapsed but unscorable eggs that were excluded from further analysis varied from 4–13 among each of the eight genotypes. Fisher’s exact test:
****: different from sibling controls, p<0.0001
*: p = 0.024.