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. 2023 Dec 4;17(6):064103. doi: 10.1063/5.0170606

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© 2023 Author(s).

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Published open access through an agreement with Politecnico di Milano18981

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FIG. 2. — HUVEC cylindrical cell monolayer generation. Time-course of fluorescently labeled HUVEC growth to form a cylindrical monolayer at (a) 24 h (b) 48 h, and (c) 72 h post-seeding. (i) Bright field and (ii) z-stacks confocal maximal projection (25×/0.95 water immersion objective) tile-scan acquisitions of the central lumen within the DC device, full length. (iii) Enlargements of (ii), z-stacks confocal maximal projection of a single tile of the central lumen within the DC device. DC device. (iv) Central lumen full length tile-scan z-stacks and (v) y-stack central lumen cross section confocal 3D renderings. Scale bar (white), 100 μm.