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. 2023 Dec 4;7(4):046116. doi: 10.1063/5.0171109

FIG. 5.

FIG. 5.

Metabolomic analysis of tumor cells treated with endothelial cell secreted factors indicate broader metabolic alterations. (a) Glucose consumption of MDA-MB-231 treated with or without HUVEC-CM for 48 h (n = 3 wells, error bar represents standard deviation). (b) Lactate production of MDA-MB-231 treated with or without HUVEC-CM for 48 h (n = 3 wells, error bar represents standard deviation). (c) Log twofold changes of the 20 amino acids of MDA-MB-231 treated with or without HUVEC-CM for 48 h relative to starting amounts at t = 0 h (n = 3 wells, error bar represents standard deviation). (d) Graphical representation of a subset of FBA model results pertaining to the bioenergetic pathways of MDA-MB-231 treated with or without HUVEC-CM for 48 h. Inset contains expanded FBA results for the TCA cycle. Metabolites highlighted in gray represent those that were measured experimentally and used to constrain FBA results. A full list of the FBA model constraints and simulated reactions can be found in supplementary material File 1. *p < 0.05, ****p < 0.0001.