FIG. 6.

Glucose restriction and ATP synthase inhibition inhibit endothelial cell-dependent tumor cell invasion. (a) Confocal projection of MDA-MB-231 invading toward an EC channel with or without oligomycin treatment for 24 h (scale bar = 200 μm). (b) Quantification of invasion distance in microfluidic devices with or without oligomycin treatment (n = 3 biological replicates, data points represent individual cells, error bars represent standard deviation). (c) Schematic demonstrating glucose restriction in microfluidic devices. (d) Confocal projection of MDA-MB-231 invading toward an EC channel with or without glucose restriction for 5 days (scale bar = 200 μm). (e) Quantification of invasion distance in microfluidic devices with or without glucose restriction (n = 3 biological replicates, data points represent individual cells, error bars represent standard deviation). ^**p < 0.01, ^****p < 0.0001.