KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| Anti-human CD163 | Cell Signaling Technology | Cat#: 93498S; RRID: AB_2800204 |
| Anti-human CD163 | BioLegend | Cat#: 333618; RRID: AB_2563094 |
| Anti-human CD163, biotin | BioLegend | Cat#: 333604; RRID: AB_1134004 |
| Anti-human PD-L1 (5H1) | Lab of Haidong Dong | PMID: 21355078 |
| Anti-human PD-L1 | Cell Signaling Technology | Cat#: 86744; RRID: AB_2800088 |
| Anti-human PD-1 | BioLegend | Cat#: 329912; RRID: AB_961417 |
| Mouse IgG isotype control | BioLegend | Cat#: 401404; RRID: RRID:AB_2801451 |
| Anti-mouse PD-L1 | BioXCell | Cat#: BE0101; RRID: AB_10949073 |
| Rat IgG isotype control | BioXCell | Cat#: BE0090; RRID: AB_1107780 |
| Anti-human active caspase-3 | BD Horizon™ | Cat#: 564094; RRID: AB_2738587 |
| Anti-human PD-L1 | BioLegend | Cat#: 329706; RRID: AB_940368 |
| Anti-human PD-L1 | BD Biosciences | Cat#: 558065; RRID: AB_647176 |
| Anti-human CD8 | eBioscience | Cat#: 48-0088-42; RRID: AB_1272062 |
| Anti-human CD4 | Biolegend | Cat#: 317416; RRID: AB_571945 |
| Anti-human PD-1 | BioLegend | Cat#: 329904; RRID: AB_940479 |
| Anti-human Ki-67 | BD Biosciences | Cat#: 561283; RRID: AB_10716060 |
| Anti-human Granzyme B | Life Technologies | Cat#: GRB04; RRID: AB_2536538 |
| Anti-mouse PD-1 | BioLegend | Cat#: 109110; RRID: AB_572017 |
| Anti-mouse Ki-67 | BioLegend | Cat#: 652420; RRID: AB_2564285 |
| Anti-mouse Granzyme B | eBioscience | Cat#: 12-8898-82; RRID: AB_10870787 |
| Anti-mouse CD8a | eBioscience | Cat#: 48-0081-82; RRID: AB_1272198 |
| Anti-human CD63 | Abcam | Cat#: ab8219; RRID: AB_306364 |
| Anti-human CD8a | Biolegend | Cat# 372902; RRID: AB_2650657 |
| Anti-human CD63 | Abcam | Cat#: ab134045; RRID: AB_2800495 |
| Anti-human CD63 | Abcam | Cat#: ab68418; RRID: AB_10563972 |
| Anti-TSG101 | Abcam | Cat#: ab125011; RRID: AB_10974262 |
| Anti-human PD-L1 | Cell Signaling Technology | Cat#: 13684S; RRID: AB_2687655 |
| Anti-MADD | Abcam | Cat#: ab134117; RRID: AB_2650580 |
| Anti-mouse PD-L1 | eBioscience | Cat#: 12-5982-82; RRID: AB_466089 |
| Anti-mouse CD163 | BioLegend | Cat#: 155302; RRID: AB_2734239 |
| Anti-human CD9 | Cell Signaling Technology | Cat#: 13403; RRID: AB_2732848 |
| Anti-GAPDH | Cell Signaling Technology | Cat#: 5174S; RRID: AB_10622025 |
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| Bacterial and virus strains | ||
|
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| StellarCompetent Cells | Takara | Cat#: 636766 |
| Lentivirus | This paper | N/A |
|
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| Biological samples | ||
|
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| Monocytes | Penn Human Immunology Core | N/A |
| CD8 cells | Penn Human Immunology Core | N/A |
| Melanoma patient samples | University of Pennsylvania Abramson Cancer Center | UPCC# 08607/IRB# 703001 |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
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| Recombinant Human IFN-γ | PeproTech | Cat#: 300-02 |
| Recombinant Murine IFN-γ | PeproTech | Cat#: 315-05 |
| Recombinant Human M-CSF | PeproTech | Cat#: AF-300-25 |
| Recombinant Murine M-CSF | PeproTech | Cat#: 315-02 |
|
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| Critical commercial assays | ||
|
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| Dynabeads Untouched Mouse CD8 Cells Kit | Invitrogen | Cat#: 11417D |
| MagniSortStreptavidin Positive Selection Beads | Invitrogen | Cat#: MSPB-6003 |
| DAB Substrate Kit | Fisher Scientific | Cat#: BD 550880; RRID:AB_2868905 |
| MaginSortTM Mouse F4/80 Positive Selection Kit | Invitrogen | Cat#: 8802-6863-74 |
|
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| Experimental models: Cell lines | ||
|
| ||
| WM9 | Chen et al.31 | N/A |
| YUMM1.7 | Wang et al.80 | N/A |
| WM9 PD-L1 KO | This paper | N/A |
| YUMM1.7 PD-L1 KO | This paper | N/A |
| WM35 | Atay et al.69 | N/A |
| WM35 cells HLA-matched TILs | Atay et al.69 | N/A |
| HEK293T | ATCC | Cat#: CRL-3216 |
| MEL624 | Chen et al.31 | N/A |
|
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| Experimental models: Organisms/strains | ||
|
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| C57BL/6 | The Jackson Laboratory | Cat#: 000664 |
| PD-L1−/−C57BL/6 | Tang et al.4 | N/A |
|
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| Oligonucleotides | ||
|
| ||
| Human PD-L1 KO sgRNA: CTTGCACTTCTGAAGAGATTGA’ | This paper | N/A |
| Murine PD-L1 KO sgRNA: GGTCCAGCTCCCGTTCTACA | This paper | N/A |
| Human RAB27A shRNA 1 GCTGCCAATGGGACAAACATA | Sung et al.96 | N/A |
| Human RAB27A shRNA 2 CAGGAGAGGTTTCGTAGCTA | Sung et al.96 | N/A |
| Murine RAB27A shRNA 1 CGAAACTGGATAA GCCAGCTA | This paper | N/A |
| Murine RAB27A shRNA 2 GACAAACATAAGCCACGCGAT | This paper | N/A |
| Human MADD shRNA 1 CCACAAGTACAAGACGCCAAT | This paper | N/A |
| Human MADD shRNA 2 CCTGAAAGTATTTGGGCTAAA | This paper | N/A |
| scrambled shRNA | Chen et al.31 | Addgene, Cat#: 1864 |
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| Recombinant DNA | ||
|
| ||
| Plasmid: Human MADDWT | This paper | N/A |
| Plasmid: Human MADD S70A | This paper | N/A |
| Plasmid: Human MADD S70D | This paper | N/A |
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| Software and algorithms | ||
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| GraphPad software | Prism 8.0 | https://www.graphpad.com/scientific/software/prism/ |
| Fiji | Schindelin et al.97 | https://imagej.net/Fiji |
| FlowJo | Version v10 | https://www.flowjo.com/solutions/flowjo/downloads |