TABLE 2.
Analysis of kinB-cat expression in P. aeruginosa strains
| Strain | Genotype | Alginate pheno- type | Amt of CAT (pg/μg of protein) from kinB-cata |
|---|---|---|---|
| FRD1 | Wild type | Alg+ | <10 |
| FRD1::pSM53 | kinB-cat | Alg+ | 438 ± 31 |
| FRD2::pSM53 | algT18 kinB-cat | Alg− | 146 ± 21 |
| FRD444::pSM53 | algB::Tn501 kinB-cat | Alg− | <10 |
| FRD444::pSM53 (pJG1) | algB::Tn501 kinB-cat (algB+) | Alg+ | <10 |
a
P. aeruginosa FRD strains containing a chromosomally integrated pSM53 (kinB-cat) were cultured to the same density in logarithmic phase and were harvested. As a measure of relative kinB transcription, cell extracts of these strains were assayed for CAT levels relative to the protein concentrations.