Fig. 2.

Label-free quantitative proteomics and confirmation by Western blotting. After 24-h incubation with/without 100 µM trigonelline (TRIG), cellular proteins were extracted and subjected to in-solution tryptic digestion, nanoLC-ESI-Qq-TOF MS/MS analysis, and label-free quantitative proteomics. (A): A Venn diagram illustrating numbers of total and differentially expressed proteins identified from control and trigonelline-treated cells. (B): Western blotting to confirm the increased level of annexin A2 and decreased level of β-actin induced by trigonelline. GAPDH served as the loading control. (C): Band intensity of each protein was quantified and normalized by that of GAPDH. Each dot represents each data value, whereas the bar indicates mean ± SD. The data were acquired from three separate experiments using independent biological samples. Significant P values are labelled.