FIG. 2.

(A) TMBZ-stained SDS-PAGE analysis of c-type cyts from various R. capsulatus NADI⁻ mutants grown on enriched MPYE medium (100 μg of membrane proteins was loaded per lane). (B) Western blot analysis with anti-CcoN (subunit I) antibodies. After SDS-PAGE (10 μg of membrane proteins per lane) and electrophoretic transfer onto an Immobilon-P membrane. CcoN was detected with horseradish peroxidase-conjugated anti-rabbit immunoglobulin G with NiCl₂-enhanced 3,3′-diaminobenzidine as the substrate. Cyts c_p and c_o are the subunits II and III of cyt cbb₃ oxidase, and cyts c₁ and c_y correspond to the cyt c₁ subunit of the bc₁ complex and the membrane-attached electron carrier c_y, respectively.