FIG. 3.

Physical and genetic map of plasmid pOX15 carrying ccoNOQP. pOX15 was constructed by ligation of the 1.3-kb BamHI fragment of pMG1 into the BamHI site of p4AIV as described in Materials and Methods. The orientation of ORF277 and ccoNOQP and the location of the mutations in different EMS-induced or constructed NADI⁻ mutants of R. capsulatus are also indicated. Km, Tn5, Δ, and TGA correspond to the kanamycin resistance gene, transposon Tn5, deletion, and stop codon, respectively; arrowheads refer to the orientation of the insertion mutations.