TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Genotype	Phenotype	Source or reference
Strains
E. coli
HB101	F−proA2 hsdS20 (rB− mB−) recA13 ara14 lacY1 galK2 rpsL20 supE44 rpsL20 supE44 proA2 xyl-5 mtl-1		38
XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdr17 supE44 relA1 lac[F′ proAB lacIqΔM15 Tn10(Tetr)]		Stratagene
R. capsulatus
MT1131a	crtD121 Rifr	Wild type (NADI+)	40
Y262		GTA overproducer	51
GK277-1	ORF277-1::kan	Cox+ NADI+	This work
GK277-2	ORF277-2::kan	Cox+ NADI+	This work
M4	ΔccoN	Cox− NADI−	29
M5	ccoNOQP qox	Cox− Qox− NADI−	29
M7G	ccoP269	Cox− NADI−	29
MR1	ccoO107	Cox− NADI−	This work
MG1	ccoP::kan	Cox− NADI−	This work
OH2	ccoP 34	Cox− NADI−	This work
DB8	ccoP::Tn5	Cox− NADI−	This work
GK32	Δ(ccoNO::kan)	Cox− NADI−	This work
BK4	ccoNOQPb	Cox− NADI−	This work
GK2	ccoNOQPb	Cox− NADI−	This work
OH1	ccoNOQPb	Cox− NADI−	This work
SS24	ccoNOQPb	Cox− NADI−	This work
SS25	ccoNOQPb	Cox− NADI−	This work
IW2	ccoNOQPb	Cox− NADI−	This work
Sev1	ccoNOQPb	Cox− NADI−	This work
Sev2	ccoNOQPb	Cox− NADI−	This work
Sev3	ccoNOQPb	Cox− NADI−	This work
Sev4	ccoNOQPb	Cox− NADI−	This work
Sev5	ccoNOQPb	Cox− NADI−	This work
BK5		Cox− NADI−	This work
SS33		Cox− NADI−	This work
SS1		Cox− NADI slow	This work
SS2		Cox− NADI slow	This work
MR2		Cox− NADI slow	This work
IJ1		Cox− NADI slow	This work
GK1		Cox− NADI−	This work
DM2		Cox− NADI slow	This work
IW3		Cox− NADI slow	This work
TP1		Cox− NADI slow	This work
TP2		Cox− NADI slow	This work
Plasmids
pRK2013		Kanr, helper	10
pRK415		Tetr	10
pRK404		Tetr	10
pBSII	pBluescript II (KS+)	Ampr	Stratagene
pXCA601	lacZ	Tetr	1
pMA117		Kanr	7
pUC4-Kixx		Kanr	Pharmacia
p5T	pRK404 with a 16.5-kb BamHI insert	Tetr	This work
p5TΔH	HindIII deletion of p5T	Tetr	This work
p4A	pRK404 with 6.0-kb BamHI insert	Tetr	This work
p4AI	XhoI deletion of p4A	Tetr	This work
p4AIK32	Kanr in XhoI site of p4AI	Kanr Tetr	This work
p4AIII	BglII deletion of p4A	Tetr	This work
p4AIIIK	Kanr in BglII site of p4AIII	Kanr Tetr	This work
p4AIV	3.4-kb BglII-BamHI fragment of p4A in pRK404	Tetr	This work
p4AXI	1.1-kb BglII-HindIII fragment of p4A in pRK404	Tetr	This work
p4AXIK	Kanr in XhoI site of p4AXI	Kanr Tetr	This work
p4AXIIK	Kanr in NruI site of p4AXI	Kanr Tetr	This work
pMG1	1.3-kb BamHI fragment of p5TΔH in pBSII	Ampr	This work
pMG1K	Kanr in BstEII site of pMG1	Kanr Tetr	This work
pMG1-H1	10-kb HindIII fragment of MG1 in pBSII	Kanr Ampr	This work
pRHK8	1.3-kb BamHI fragment of p5TΔH in pRK404, opposite orientation from that in p5TΔH	Tetr	This work
pRHK9	1.3-kb BamHI fragment of p5TΔH in pRK404, same orientation as that in p5TΔH	Tetr	This work
pOX15	1.3-kb BamHI fragment of p5TΔH cloned into BamHI-digested p4AIV	Tetr	This work
pXG2	ORF277-ccoN::lacZ	Tetr	This work
pBK1	4.5-kb HindIII fragment in pRK404, complementing BK5	Tetr	This work
pMRC	6.0-kb EcoRI fragment in pLAFR1, complementing MR2 and IJ1	Tetr	This work
pS33	10-kb BamHI fragment in pRK404, complementing SS33, SS1, and SS2	Tetr	This work

^aR. capsulatus MT1131 (Rif^r crtD) is referred to as “wild type,” since it is wild type with respect to its cyt c profile and growth properties. MT1131 was originally isolated as a green derivative of R. capsulatus SB1003 (40). 

^bUncharacterized mutations in ccoNOQP.