TABLE 2.
Phenotypic and genetic characterization of NADI− mutants of R. capsulatusa
| Mutantb | Complementationc
|
Detection by anti-ccoN antibodies | cyt c profiled
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p5TΔH | p4A | pOX15 | pBK1 | pS33 | pMRC | cp | c1 | cy | co | c2 | ||
| Class I | ||||||||||||
| M4 | − | + | + | − | − | − | − | − | + | + | − | + |
| BK4 | − | + | + | − | − | − | − | − | + | + | − | + |
| GK2 | − | − | + | − | − | − | NTe | − | + | + | − | + |
| MR1 | − | − | + | − | − | − | − | − | + | + | − | + |
| M7G | + | − | + | − | − | − | + | − | + | + | + | + |
| MG1 | + | − | + | − | − | − | − | − | + | + | − | + |
| OH2 | + | − | + | − | − | − | − | − | + | + | − | + |
| DB8 | + | − | + | − | − | − | − | − | + | + | − | + |
| GK32 | − | − | + | − | − | − | − | − | + | + | − | + |
| Class II; BK5 | − | − | − | + | − | − | + | + | + | + | + | + |
| Class III | ||||||||||||
| SS33 | − | − | − | − | + | − | − | − | + | + | − | + |
| SS1f | − | − | − | − | + | − | (−) | (−) | + | + | (−) | + |
| SS2f | − | − | − | − | + | − | (−) | (−) | + | + | (−) | + |
| Class IV | ||||||||||||
| MR2g | − | − | − | − | − | + | (+) | (+) | (+) | − | (+) | (+) |
| IJ1g | − | − | − | − | − | + | (+) | (+) | (+) | − | (+) | (+) |
a
−, negative; +, positive.
b
In addition to the mutants listed above, OH1, SS24, SS25, IW2, Sev1, Sev2, Sev3, Sev4, and Sev5 were also classified as class I mutants, since they were complemented by pOX15.
c
For a detailed description of the complementing plasmids, see the text.
d
Based on TMBZ-stained SDS-PAGE.
e
NT, not tested.
f
Traces of CcoN, CcoP, and CcoO [(−)] were detectable.
g
Small amounts of CcoN, CcoP, and CcoO [(+)] were detectable.