TABLE 3.
TMPD oxidase activities in chromatophores from various R. capsulatus mutants grown chemoheterotrophically in MPYE medium
| Strain | Sp act (μmol of O2/h/mg of protein)a
|
% of wild-type activity | |
|---|---|---|---|
| Ascorbate-TMPD | + KCN | ||
| MT1131 | 34.47 | 0.98 | 100 |
| MT1131/pOX15 | 183.6 | 0.2 | 532 |
| GK277-1 | 31.4 | 0.7 | 91 |
| GK277-2 | 28.8 | 0.2 | 83.6 |
| Mutants | |||
| Class I | |||
| GK32 | 0.5 | 0.5 | 0 |
| M4 | 0.6 | 0.45 | 1.8 |
| BK4 | 0 | 0 | 0 |
| MR1 | 1.6 | 0.38 | 1.1 |
| DB8 | 0.54 | 0.54 | 0 |
| OH2 | 1.2 | 0.83 | 1.1 |
| MG1 | 0.2 | 0.1 | 0.3 |
| M7G | 0.97 | 0.24 | 2.1 |
| Class II, BK5 | 0.9 | 0.65 | 0.73 |
| Class III | |||
| SS33 | 0.3 | 0.11 | 0.6 |
| SS1 | 1.5 | 0.15 | 3.9 |
| SS2 | 1.6 | 0.25 | 3.9 |
| Class IV | |||
| MR2 | 6.5 | 0.8 | 16.6 |
| IJ1 | 7.2 | 0.6 | 19.2 |
| Unclassified | |||
| GK1 (MPYE) | 0.8 | 0.35 | 1.3 |
| GK1 (Med A) | 5.6 | 0.21 | 16 |
| IW3 | 1.7 | 0.25 | 4.0 |
a
Values shown are means of at least three independent measurements. Note that the activity found in GK1 is dependent on the growth medium.