Table 1.

Test information and results

Test	Purpose of the test	Validation of the result
		Positive	Negative
Indole test	Screen for the organism that degrades tryptophan and produces indole	Cherry red ring formation	Yellow/no ring formation
Methyl red	Detects if the organism performs mixed acid fermentation	Red	Yellow
Voges Proskauer	Detects the presence of acetoin within the metabolic pathway	Red/purple color	No color
Citrate utilization	Screen for the organism that uses citrate as carbon and energy source	Blue	Green
Catalase	Used for the detection of enzyme catalase	Effervescence	No effervescence
Coagulase	Used for the detection of protein coagulase	Clot formation	No clot
Oxidase	Assay for the presence of cytochrome oxidase	Purple	Yellow/no color
Urease	To test the ability of microbe that degrades urea	Pink	Yellow
Triple sugar iron agar	Determination of carbohydrate fermentation and H2S production	Pink with gas	Yellow
Blood hemolysis	Test for the ability of organism that produces haemolysin	Growth seen	No growth
Starch hydrolysis	Test for the ability of organism that hydrolyses starch	Blue with zone of inhibition	No zone
Organic acid production	Detection of microbe if it produces any organic acid as an intermediate	Thick white colony growth	Pale /transparent growth