Fig. 1. HylA enzyme is a major virulence factor in acne pathogenesis.
a pie chart showing health-and acne-associated C. acnes phylotypes and association with hylA or hylB gene6,13. b–f CD1 mice (n = 10) were infected intradermally (i.d.) with 2x107CFU WT (HL043PA1 or HL110PA3) or isogenic mutant (∆hylA or ∆hylB) C. acnes, followed by topical application of sebum daily. Bacterial burden (b), disease score (c), and cytokines (d–f) at 2 d (48 h) post-infection. g–j CD1 mice (n = 10) were infected as above with either HL043PA1, ∆hylA or ∆hylA plus recombinant (r) HylA protein (10 μg). Disease score (g), and tissue cytokines (h, j) at 2d post-infection. b–j Data were from two independent experiments with each data point representing one mouse. Bars denote median. The data in b, c and e–i were analyzed by one-way ANOVA with Tukey’s post-hoc test. The data in d and j were analyzed by non-parametric Kruskal-Wallis one-way ANOVA test. Source data are provided as a Source Data file.