TABLE 1.
E. coli strains used in this study
| Straina (H anti- gen expressed) | Flagellar genotypeb | Other relevant markers | Origin (reference[s])c |
|---|---|---|---|
| 781-55 (H44) | fliC4offfllA44on | A (19) | |
| E2987-73 (H55) | fliC38offfllA55on | A (19) | |
| E223-69 (H54) | flmA54on | A (19) | |
| EJ34 (NM) | fliCiah-1 | E. coli K-12 derivative (7) | |
| EJ262 (e,n,x) | fliCiah-1 hin+ fljBe,n,xfljA+ | Salmonella phase 2 flagellin region transduced into EJ34 (6) | |
| PM298 (H48) | fliC48on | Strr RifrproA23 trp-30 his-51 | E. coli K-12 derivative (23) |
| PM304 (H44) | fliC4offfllA44on | Strr RifrproA23 trp-30 | A; PM298 with flagellin genes received from F′ variant of strain 781-55 |
| PM315 (H3) | fliCiah-1 flkA3on | EJ34 with flkA3 introduced (23) | |
| PM335 (H48) | fliC48on | Nalr Rifrstr-104 leuB6 hisG1 argG6 metB1 malT1 xyl-7 mtlA2 | E. coli K-12 derivative (23) |
| PM336 (H16) | fliC16on | Same as in PM335 | E. coli K-12 derivative (23) |
| PM342 (H16) | fliC16on | str-3 hisG1 purF pheA2 argH mtlA2 malT1 xyl-7 | E. coli K-12 derivative (23) |
| PM470 (H44) | fliCiah-1 fllA44on | A; EJ34 with fllA44 transduced from PM304 | |
| PM471 (H55) | fliCiah-1 fllA55on | A; EJ34 with fllA55 transduced from E2987-69 | |
| PM472 (H54) | fliCiah-1 flmA54on | A; EJ34 with flmA transduced from E223-69 | |
| PM476F′ (H55) | fliCiah-1 fllA55on | A; PM471 with sex factor F′ Lac+ Kmr introduced | |
| PM479a (H54) | fliC16offflmA54on | Δ(pro-lac) thi-1 | A; Hfr-donord |
| PM479b (H54) | fliC16offflmA54on | Δ(pro-lac) thi-1 | A; Hfr-donord |
| B99-2 (H6) | fliC6on | (20, 21) |
a
In strain EJ34, native E. coli K-12 flagellin gene fliC48 was replaced with salmonella phase 1 flagellin gene fliCi (previously named H1) altered by the mutation ah-1 and unable to produce flagellin; therefore, the strain was nonflagellated and NM, although all of the other flagellar genes were intact (7).
b
Superscripts: off, latent state; on, expressed state of a flagellin gene.
c
A, flagellar genotype determined during this study.
d
Hfr donors PM479a and PM479b were similar but produced separately during this study from E. coli K-12 Hfr strain KL191 (13) by substituting its fliC48 (repressor-insensitive allele) for fliC16 (repressor-sensitive allele) and then introducing flmA54.